An highly processive, recombinant (from E.coli strain), thermostable DNA with a very high efficiency of 5’- 3’ polymerase activity and 3´- 5’ exonuclease (non-proofreading) activity. Xtra Taq Pol catalyzes the addition of mononucleotide units to the 3’-end of a primer chain, leading to the formation of DNA products that have 3’-overhanging A nucleotides (thus can be used in TA cloning). This enzyme remains funtional even after prolonged incubation steps at 95°C. The enzyme is supplied at 5U/μl and comes with 5X XtraWhite new buffer.

5X XtraWhite is a Genespin proprietary formulation, developed for standard and/or high-fidelity amplification of high-GC (>75%) templates. The buffer contains 7.5mM magnesium, PCR enhancers and thickening agents (vortex thoroughly prior to use) and is supplied with 1ml of 6X Orange Loading Dye.

The Orange G dye migrates at the same rate as a duplex DNA fragment of approximately 50 Kbp and does not interfere with DNA migration when it is used as a loading dye for agarose gel electrophoresis.

©2007-2018 GeneSpin Srl

You are here: molecular biology / endpoint PCR / XtraTaq Pol White

endpoint PCR

Taq Polymerase

cat. no.

amount

note

XSTS-T5XW 250

XSTS-T5XW 1000

250 units

5X XtraWhite Buffer

5X XtraWhite Buffer

.PDFMB_end_point_XtraTaq_W_files/StoS%20XtraTaq%20White%20ds.pdf

XSTS-T5XWw 250

XSTS-T5XWw 1000

1000 units

250 units

5X XtraWhite Buffer w/o MgCl2

5X XtraWhite Buffer w/o MgCl2

cat. no.

amount

note

XSTSn-T5XW 250

XSTSn-T5XW 1000

1000 units

250 units

5X XtraWhite Buffer + dNTPs

5X XtraWhite Buffer + dNTPs

XSTSn-T5XWw 250

XSTSn-T5XWw 1000

1000 units

250 units

5X XtraWhite Buffer w/o MgCl2 + dNTPs

5X XtraWhite Buffer w/o MgCl2 + dNTPs

add nucleotides box

FOR RESEARCH USE ONLY

UNIT DEFINITION

One unit is defined as the amount of enzyme required to incorporate 10 nanomoles of dNTPs into acid-insolubile material in 30 min at 74°C.

SHIPPING

Shipped in green ice.

STORAGE

Store at -20C°

SHELF LIFE

12 months

FORM

Liquid

CONCENTRATION

5U/ul

component

XSTS-T5XW 250

XSTS-T5XWw 250

XSTSn-T5XW 250

XSTSn-T5XWw 250

XtraTaq Polymerase

White Buffer

MgCl2

dNTPs

250 units / 50ul

1.5ml 5X XtraWhite Buffer

with MgCl2

250 units / 50ul

250 units / 50ul

250 units / 50ul

-

-

100ul / 10mM each

100ul / 10mM each

-

500ul / 50mM

-

500ul / 50mM

component

XSTS-T5XW 1000

XSTS-T5XWw 1000

XSTSn-T5XW 1000

XSTSn-T5XWw 1000

XtraTaq Polymerase

White Buffer

MgCl2

dNTPs

1000 units / 200ul

1000 units / 200ul

1000 units / 200ul

1000 units / 200ul

-

500ul / 50mM

-

500ul / 50mM

Assay Set-Up:

Before starting, vortex all components thoroughly to ensure homogeneity.

Prepare a premix for the number of assays you need according to the following protocol:

component

5X Buffer

dNTPs

XtraTaq Polymerase

primers

1000 units

DNA Template

MG Water

stock conc.

5X

10mM each

5U/ul

1ug/ul each

-

-

final conc.

1X

200uM

0.025U/ul

50ng/ul each

-

-

30ul reaction

6.0ul

0.6ul

0.2ul

2.0ul each

10-20ng

up to 30ul

> XtraTaq Pol White

Cycling conditions:

Spin down the tubes/plate briefly to remove bubbles and place them into the cycler.

denaturation

denaturation (1)

annealing (2)

extension

95°C

95°C

50-68°C

72°C

5min

30 sec

30 sec

30 sec

4 min

20-35x
1x

1)The annealing temperature depends on the melting temperature of the primers used.

2)The elongation time depends on the length of the fragments to be amplified. A time

of 1 min/kb is recommended.

1.5ml 5X XtraWhite Buffer

w/o MgCl2

1.5ml 5X XtraWhite Buffer

with MgCl2

1.5ml 5X XtraWhite Buffer

w/o MgCl2

4ml 5X XtraWhite Buffer

with MgCl2

4ml 5X XtraWhite Buffer

w/o MgCl2

4ml 5X XtraWhite Buffer

with MgCl2

4ml 5X XtraWhite Buffer

w/o MgCl2

-

-

400ul / 10mM each

400ul / 10mM each

.PDFMB_end_point_XtraTaq_W_files/StoS%20XtraTaq%20White%20ds_1.pdf

*5X XtraWhite Buffer is supplied with appropriate quantity of 6X Loading Dye

*5X XtraWhite Buffer is supplied with appropriate quantity of 6X Loading Dye

1.5ml 5X Buffer with MgCl2

GeneSpin Srl

Via Friuli, 51

20135 Milano

P.IVA C.F. 04520270960

Laboratory

and sales office

c/o Fondazione Filarete

Viale Ortles, 22/4

20139 Milano

tel. +39 02 56660199

fax. +39 02 537250

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