Taq Pol is a highly processive, recombinant (isolated and purified from an E. coli strain), thermostable DNA polymerase with 5´→ 3´ polymerase activity, which catalyzes the addition of mononucleotide units to the 3’-OH end of a primer chain. This enzyme remains functional even after prolonged incubation steps at 95°C.

©2007-2018 GeneSpin Srl

©2007-2018 GeneSpin Srl

You are here: molecular biology / endpoint PCR / Taq Pol

endpoint PCR

Taq Polymerase

cat. no.

amount

note

STS-T250

STS-T1000

250 units

10X Standard Buffer

10X Standard Buffer

.PDFMB_end_point_Taq_files/StoS%20Taq%20Pol%20ds.pdf

STS-Tw250

STS-Tw1000

1000 units

250 units

10X Standard Buffer w/o MgCl2

10X Standard Buffer w/o MgCl2

cat. no.

amount

note

STSn-T250

STSn-T1000

1000 units

250 units

10X Standard Buffer + dNTPs

10X Standard Buffer + dNTPs

STSn-Tw250

STSn-Tw1000

1000 units

250 units

10X Standard Buffer w/o MgCl2 + dNTPs

10X Standard Buffer w/o MgCl2 + dNTPs

add nucleotides box

FOR RESEARCH USE ONLY

UNIT DEFINITION

One unit is defined as the amount of enzyme required to incorporate 10 nanomoles of dNTPs into acid-insolubile material in 30 min at 74°C.

SHIPPING

Shipped in green ice.

STORAGE

Store at -20C°

SHELF LIFE

12 months

FORM

Liquid

CONCENTRATION

5U/ul

component

STS-T250

STS-Tw250

STSn-T250

STSn-Tw250

Taq Polymerase

Standard Buffer

MgCl2

dNTPs

250 units / 50ul

1ml 10X Buffer with MgCl2

1ml 10X Buffer w/o MgCl2

1ml 10X Buffer with MgCl2

1ml 10X Buffer w/o MgCl2

250 units / 50ul

250 units / 50ul

250 units / 50ul

-

-

100ul / 10mM each

100ul / 10mM each

-

500ul / 50mM

-

500ul / 50mM

component

STS-T1000

STS-Tw1000

STSn-T1000

STSn-Tw1000

Taq Polymerase

Standard Buffer

MgCl2

dNTPs

1000 units / 200ul

2ml 10X Buffer with MgCl2

2ml 10X Buffer w/o MgCl2

2ml 10X Buffer with MgCl2

2ml 10X Buffer w/o MgCl2

1000 units / 200ul

1000 units / 200ul

1000 units / 200ul

-

-

400ul / 10mM each

400ul / 10mM each

-

500ul / 50mM

-

500ul / 50mM

Assay Set-Up:

Before starting, vortex all components thoroughly to ensure homogeneity.

Prepare a premix for the number of assays you need according to the following protocol:

component

Standard Buffer

dNTPs

Taq Polymerase

primers

1000 units

DNA Template

MG Water

stock conc.

10X

10mM each

5U/ul

1ug/ul each

-

-

final conc.

1X

200uM

0.025U/ul

50ng/ul each

-

-

30ul reaction

3.0ul

0.6ul

0.2ul

2.0ul each

10-20ng

up to 30ul

> Taq Pol

Cycling conditions:

Spin down the tubes/plate briefly to remove bubbles and place them into the cycler.

denaturation

denaturation (1)

annealing (2)

extension

95°C

95°C

50-68°C

72°C

5min

30 sec

30 sec

30 sec

4 min

20-35x
1x

1)The annealing temperature depends on the melting temperature of the primers used.

2)The elongation time depends on the length of the fragments to be amplified. A time

of 1 min/kb is recommended.

10X BUFFER

10 x conc. complete PCR buffer containing 100 mM Tris-HCl, KCl, and 15 mM MgCl2.

.PDFMB_end_point_Taq_files/StoS%20Taq%20Pol%20ds_1.pdf

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