2X XtraWhite Master Mix and 2X XtraRTL Master Mix (Genespin proprietary formulation), are two premixed, ready-to-use solution containing Xtra-Taq Pol, dNTPs and MgCl2 in a Reaction Buffer optimized for use in PCR amplification of targets present in low copy number and to avoid amplification of non-specific products. Both buffers contain 3.0mM magnesium, PCR enhancers and thickening agents (vortex thoroughly prior to use).

2X XtraRTL Master Mix contains Orange G dye that allows gel loading and electrophoresis of the sample directly from the PCR tube, without further manipulation. 2X XtraWhite Master Mix is supplied with appropriate quantity of 6X Loading Dye. The Orange G dye migrates at the same rate as a duplex DNA fragment of approximately 50 Kbp and does not interfere with DNA migration when it is used as a loading dye for agarose gel electrophoresis.

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You are here: molecular biology / endpoint PCR /  2X Master Mixes Standard

endpoint PCR

Master mixes

cat. no.

amount

note

STS-XMMixW 200*

STS-XMMixW 1000*

5ml

2X XtraWhite Master Mix

2X XtraWhite Master Mix

.PDFMB_end_point_MMIX_Standard_files/StoS%202X%20Master%20Mix%20Standard%20ds.pdf

STS-XMMixRTL 200

STS-XMMixRTL 1000

25ml

5ml

2X XtraRTL Master Mix

2X XtraRTL Master Mix

FOR RESEARCH USE ONLY

SHIPPING

Shipped in green ice.

STORAGE

Store at -20C°. Avoid freeze/thaw cycles.

SHELF LIFE

12 months

FORM

Liquid

CONCENTRATION

2X conc.

Assay Set-Up:

Before starting, vortex all components thoroughly to ensure homogeneity.

Prepare a premix for the number of assays you need according to the following protocol:

component

2X Master Mix

primers

DNA Template

MG Water

25ml

stock conc.

2X

1ug/ul each

-

-

final conc.

1X

50ng/ul each

-

-

30ul reaction

15.0ul

2.0ul each

10-20ng

up to 30ul

> 2X Master mix Standard

Cycling conditions:

Spin down the tubes/plate briefly to remove bubbles and place them into the cycler.

denaturation

denaturation (1)

annealing (2)

extension

95°C

95°C

50-68°C

72°C

5min

30 sec

30 sec

30 sec

4 min

20-35x
1x

1)The annealing temperature depends on the melting temperature of the primers used.

2)The elongation time depends on the length of the fragments to be amplified. A time

of 1 min/kb is recommended.

*2X XtraWhite Master Mix is supplied with appropriate quantity of 6X Loading Dye

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