2X Hot Start XtraWhite Master Mix and 2X Hot Start XtraRTL Master Mix (Genespin proprietary formulation), are two premixed, ready-to-use solution containing Hot Taq Pol, dNTPs and MgCl2 in a Reaction Buffer optimized for use in PCR amplification of targets present in low copy number and to avoid amplification of non-specific products. Both buffers contain 3.0mM magnesium, PCR enhancers and thickening agents (vortex thoroughly prior to use).
Both Master Mixes allow for specific PCR amplification by keeping the enzyme inactive until the temperature reaches approximately 40°C, while also reducing samples preparation time as well as risk of contamination from multiple pipetting steps.
2X Hot Start XtraRTL Master Mix contains Orange G dye that allows gel loading and electrophoresis of the sample directly from the PCR* tube, without further manipulation. 2X Hot Start XtraWhite Master Mix is supplied with appropriate quantity of 6X Loading Dye. The Orange G dye migrates at the same rate as a duplex DNA fragment of approximately 50 Kbp and does not interfere with DNA migration when it is used as a loading dye for agarose gel electrophoresis.
