2X Quantitative Master Mix SYBR Green, is a 2X premixed, ready-to-use solution (Genespin proprietary formulation) containing Xtra Taq Pol, dNTPs, MgCl2 and stabilizers optimized for use in real time PCR amplification.
2X Quantitative Master Mix SYBR Green contains fluorescein for dynamic well factor collection. The addiction of fluorescein has no effect on the PCR reaction efficiency or sensitivity of detection.
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2X Quantitative Master Mix
cat. no.
amount
note
QSTS-SMMix200
QSTS-SMMix500
5ml
2X conc.
2X conc.
QSTS-SMMix1000
25ml
2X conc.
FOR RESEARCH USE ONLY
SHIPPING
Shipped in green ice.
STORAGE
Store at -20C°
avoid freeze/thaw cycles, store dark
Storage at 4 °C for up to 3 months possible.
SHELF LIFE
12 months
FORM
Liquid
CONCENTRATION
2X
12.5ml
MG Water
-
-
> 2X Quantitative Master Mix with SYBR® Green
qPCR
Assay Set-Up:
Before starting, vortex all components thoroughly to ensure homogeneity.
Prepare a premix for the number of assays you need according to the following protocol:
component
2X Master Mix
primer for
primer rev
DNA Template
stock conc.
2X
10uM
10uM
-
final conc.
1X
100-400 nM
100-400 nM
-
20ul reaction
10.0ul
0.2 - 0.8 ul
0.2 - 0.8 ul
< 500ng
Cycling conditions:
Spin down the tubes/plate briefly to remove bubbles and place them into the cycler.
denaturation
denaturation (1)
annealing (2) and extension
95°C
95°C
55-68°C
5min
10 sec
30 sec
1)The annealing temperature depends on the melting temperature of the primers used.
2)The elongation time depends on the length of the fragments to be amplified. A time
of 1 min/kb is recommended.
50ul reaction
25.0ul
0.5 - 2.0 ul
0.5 - 2.0 ul
<500ng
MG Water
-
-
up to 20ul
up to 50ul
melt curve
from 65°C to 95°C
0.5°C/5sec
SYBR® is a registered trademark of Invitrogen Corporation, Carlsbad, California, USA
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