Taq Pol is a highly processive, recombinant (isolated and purified from an E. coli strain), thermostable DNA polymerase with 5´→ 3´ polymerase activity, which catalyzes the addition of mononucleotide units to the 3’-OH end of a primer chain. This enzyme remains functional even after prolonged incubation steps at 95°C.
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endpoint PCR
Taq Polymerase
cat. no.
amount
note
STS-T250
STS-T1000
250 units
10X Standard Buffer
10X Standard Buffer
STS-Tw250
STS-Tw1000
1000 units
250 units
10X Standard Buffer w/o MgCl2
10X Standard Buffer w/o MgCl2
cat. no.
amount
note
STSn-T250
STSn-T1000
1000 units
250 units
10X Standard Buffer + dNTPs
10X Standard Buffer + dNTPs
STSn-Tw250
STSn-Tw1000
1000 units
250 units
10X Standard Buffer w/o MgCl2 + dNTPs
10X Standard Buffer w/o MgCl2 + dNTPs
add nucleotides box
FOR RESEARCH USE ONLY
UNIT DEFINITION
One unit is defined as the amount of enzyme required to incorporate 10 nanomoles of dNTPs into acid-insolubile material in 30 min at 74°C.
SHIPPING
Shipped in green ice.
STORAGE
Store at -20C°
SHELF LIFE
12 months
FORM
Liquid
CONCENTRATION
5U/ul
component
STS-T250
STS-Tw250
STSn-T250
STSn-Tw250
Taq Polymerase
Standard Buffer
MgCl2
dNTPs
250 units / 50ul
1ml 10X Buffer with MgCl2
1ml 10X Buffer w/o MgCl2
1ml 10X Buffer with MgCl2
1ml 10X Buffer w/o MgCl2
250 units / 50ul
250 units / 50ul
250 units / 50ul
-
-
100ul / 10mM each
100ul / 10mM each
-
500ul / 50mM
-
500ul / 50mM
component
STS-T1000
STS-Tw1000
STSn-T1000
STSn-Tw1000
Taq Polymerase
Standard Buffer
MgCl2
dNTPs
1000 units / 200ul
2ml 10X Buffer with MgCl2
2ml 10X Buffer w/o MgCl2
2ml 10X Buffer with MgCl2
2ml 10X Buffer w/o MgCl2
1000 units / 200ul
1000 units / 200ul
1000 units / 200ul
-
-
400ul / 10mM each
400ul / 10mM each
-
500ul / 50mM
-
500ul / 50mM
Assay Set-Up:
Before starting, vortex all components thoroughly to ensure homogeneity.
Prepare a premix for the number of assays you need according to the following protocol:
component
Standard Buffer
dNTPs
Taq Polymerase
primers
1000 units
DNA Template
MG Water
stock conc.
10X
10mM each
5U/ul
1ug/ul each
-
-
final conc.
1X
200uM
0.025U/ul
50ng/ul each
-
-
30ul reaction
3.0ul
0.6ul
0.2ul
2.0ul each
10-20ng
up to 30ul
> Taq Pol
Cycling conditions:
Spin down the tubes/plate briefly to remove bubbles and place them into the cycler.
denaturation
denaturation (1)
annealing (2)
extension
95°C
95°C
50-68°C
72°C
5min
30 sec
30 sec
30 sec
4 min
1)The annealing temperature depends on the melting temperature of the primers used.
2)The elongation time depends on the length of the fragments to be amplified. A time
of 1 min/kb is recommended.
10X BUFFER
10 x conc. complete PCR buffer containing 100 mM Tris-HCl, KCl, and 15 mM MgCl2.
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molecular
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