GeneSpin Srl

Via Friuli, 51

20135 Milano

P.IVA C.F. 04520270960

Laboratory 

and sales office

c/o Fondazione Filarete

Viale Ortles, 22/4

20139 Milano

tel. +39 02 56660199

fax. +39 02 537250

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High Fidelity PCR and Cloning

Reverse Transcription

Quantitative PCR

Endpoint PCR Green Line

> XtraTaq Pol RTL

An highly processive, recombinant (from E.coli strain), thermostable DNA with a very high efficiency of 5’- 3’ polymerase activity and 3´- 5’ exonuclease (non-proofreading) activity. Xtra Taq Pol catalyzes the addition of mononucleotide units to the 3’-end of a primer chain, leading to the formation of DNA products that have 3’-overhanging A nucleotides (thus can be used in TA cloning). This enzyme remains funtional even after prolonged incubation steps at 95°C. The enzyme is supplied at 5U/μl and comes with 5X XtraRTL (Ready To Load) new buffer.

5X XtraRTL Reaction Buffer is a GeneSpin proprietary formulation, developed for standard and/or high-fidelity amplification of high-GC (>75%) templates. The buffer contain 7.5mM magnesium, PCR enhancers and thickening agents (vortex thoroughly prior to use).

5X XtraRTL Reaction Buffer contains Orange G dye that allows gel loading and electrophoresis of the sample directly from the PCR tube, without further manipulation. The Orange G dye migrates at the same rate as a duplex DNA fragment of approximately 50 Kbp and does not interfere with DNA migration when it is used as a loading dye for agarose gel electrophoresis.

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