T4 DNA Ligase (isolated and purified from an E. coli strain) catalyzes the formation of a phosphodiester bond between juxtaposed5’-phosphate and 3’-hydroxyl groups in duplex DNA or RNA. This enzyme is capable of joining adjacent nucleotides in either a blunt-ended or cohesive ended configuration, as well as repairing single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.

cat. no.

amount

note

PDF

STS-MSXw10

100ul

M-MLV in different tube, w/o RNase

FOR RESEARCH USE ONLY

STS-MSXw100

4 x 100ul

M-MLV in different tube, w/o RNase

SHIPPING

Shipped in green ice.

STORAGE

Store at -20C°

SHELF LIFE

12 months

FORM

Liquid

CONCENTRATION

5X M-MLV Super Mix, 200U/ul M-MLV Reverse Transcriptase (cat no. STS-MRT)

component

STS-MSXw10

STS-MSXw100

5X M-MLV Buffer

M-MLV Reverse Transcriptase

100ul / 25 reactions

200U/ul / 25ul

4x100ul / 100 reactions

200U/ul / 100ul

RNase free Water

1ml 

1ml 

RNase

-

-

First Strand cDNA synthesis

Assay Set-Up:

Before starting, vortex all components thoroughly to ensure homogeneity.

Prepare a premix for the number of assays you need according to the following protocol:

component

stock conc.

final conc.

20ul reaction

5X M-MLV Buffer

5X

1X

4.0ul

M-MLV Reverse Transcriptase

200U/ul

100-200U

0.5-1ul

RNA

-

-

1ng to 4ug

nuclease free water

-

-

up to 20ul

First Strand cDNA synthesis

Protocol:

Spin down the tubes/plate briefly to remove bubbles.

25°C

5 min

42°C

30 min 

85°C

5 min

- Incubate @ 42°C for 60 - 120 min.

- Inactivate the reaction @ 70°C for 10 min.



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