MMLV Mix 5X is is a premixed, ready-to-use reagent (GeneSpin proprietary formulation) containing, dNTPs, MgCl2 , DDT, oligo d(T), random primers RNase Inhibitor and stabilizers optimized for first strand cDNA synthesis. M-MLV Reverse Transcriptase is not supplied directly in the SuperMix 5X but in a different tube.

The MMLV Mix 5X composition is particularly recommended for gene expression analysis in RT-PCR and Real time RT-qPCR.

cat. no.

amount

note

PDF

STS-MSX10

100ul

M-MLV in different tube, with RNase

FOR RESEARCH USE ONLY

STS-MSX100

4 x 100ul

M-MLV in different tube, with RNase

SHIPPING

Shipped in green ice.

STORAGE

Store at -20C°

SHELF LIFE

12 months

FORM

Liquid

CONCENTRATION

5X M-MLV Super Mix, 200U/ul M-MLV Reverse Transcriptase (cat no. STS-MRT)

component

STS-MSX10

STS-MSX100

5X M-MLV Buffer

M-MLV Reverse Transcriptase

100ul / 25 reactions

200U/ul / 25ul

4x100ul / 100 reactions

200U/ul / 100ul

RNase free Water

1ml 

1ml 

RNase

in the 5X M-MLV Buffer tube

in the 5X M-MLV Buffer tube

First Strand cDNA synthesis

Assay Set-Up:

Before starting, vortex all components thoroughly to ensure homogeneity.

Prepare a premix for the number of assays you need according to the following protocol:

component

stock conc.

final conc.

20ul reaction

5X M-MLV Buffer

5X

1X

4.0ul

M-MLV Reverse Transcriptase

200U/ul

100-200U

0.5-1ul

RNA

-

-

1ng to 4ug

nuclease free water

-

-

up to 20ul

First Strand cDNA synthesis

Protocol:

Spin down the tubes/plate briefly to remove bubbles.

25°C

5 min

42°C

30 min 

85°C

5 min

- Incubate @ 42°C for 60 - 120 min.

- Inactivate the reaction @ 70°C for 10 min.



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