GeneSpin Srl
Via Friuli, 51
20135 Milano
P.IVA C.F. 04520270960
Laboratory
and sales office
c/o Fondazione Filarete
Viale Ortles, 22/4
20139 Milano
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fax. +39 02 537250
2X Quantitative Master Mix SYBR Green, is a 2X premixed, ready-to-use solution (GeneSpin proprietary formulation) containing Xtra Taq Pol, dNTPs, MgCl2 and stabilizers optimized for use in real time PCR amplification.
Endpoint PCR
qPCR Master Mix with SYBR Green
qPCR Master Mix for Probes
QSTS-RMMix200
QSTS-RMMix500
QSTS-RMMix1000
5ml
2X conc.
12.5ml
2X conc.
25ml
2X conc.
FOR RESEARCH USE ONLY
SHIPPING
Shipped in green ice.
STORAGE
Store at -20C°
avoid freeze/thaw cycles, store dark
Storage at 4 °C for up to 3 months possible.
SHELF LIFE
12 months
FORM
Liquid
CONCENTRATION
2X
Assay Set-Up:
Before starting, vortex all components thoroughly to ensure homogeneity.
Prepare a premix for the number of assays you need according to the following protocol:
component
stock conc.
final conc.
20ul reaction
50ul reaction
2X Master Mix
2X
1X
10.0ul
25.0ul
primer for
10uM
100-400 nM
0.2 - 0.8 ul
0.5 - 2.0 ul
primer rev
10uM
100-400 nM
0.2 - 0.8 ul
0.5 - 2.0 ul
probes
10uM
200 nM
0.4ul
1ul
DNA Template
-
-
< 500ng
<500ng
MG Water
-
-
up to 20ul
up to 50ul
Cycling conditions:
Spin down the tubes/plate briefly to remove bubbles and place them into the cycler.
denaturation
95°C
5min
denaturation (1)
annealing (2) and extension
95°C
55-68°C
10 sec
30 sec
1)The annealing temperature depends on the melting temperature of the primers used.
2)The elongation time depends on the length of the fragments to be amplified. A time
of 1 min/kb is recommended.
SYBR® is a registered trademark of Invitrogen Corporation, Carlsbad, California, USA
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