GeneSpin Srl
Via Friuli, 51
20135 Milano
P.IVA C.F. 04520270960
Laboratory
and sales office
c/o Fondazione Filarete
Viale Ortles, 22/4
20139 Milano
tel. +39 02 56660199
fax. +39 02 537250
2X Quantitative Master Mix SYBR Green, is a 2X premixed, ready-to-use solution (GeneSpin proprietary formulation) containing Xtra Taq Pol, dNTPs, MgCl2 and stabilizers optimized for use in real time PCR amplification.
Endpoint PCR
QSTS-MMix200
QSTS-MMix500
QSTS-MMix1000
5ml
2X conc.
12.5ml
2X conc.
25ml
2X conc.
FOR RESEARCH USE ONLY
SHIPPING
Shipped in green ice.
STORAGE
Store at -20C°
avoid freeze/thaw cycles, store dark
Storage at 4 °C for up to 3 months possible.
SHELF LIFE
12 months
FORM
Liquid
CONCENTRATION
2X
Assay Set-Up:
Before starting, vortex all components thoroughly to ensure homogeneity.
Prepare a premix for the number of assays you need according to the following protocol:
component
stock conc.
final conc.
20ul reaction
50ul reaction
2X Master Mix
2X
1X
10.0ul
25.0ul
primer for
10uM
100-400 nM
0.2 - 0.8 ul
0.5 - 2.0 ul
primer rev
10uM
100-400 nM
0.2 - 0.8 ul
0.5 - 2.0 ul
probes
10uM
200 nM
0.4ul
1ul
DNA Template
-
-
< 500ng
<500ng
MG Water
-
-
up to 20ul
up to 50ul
Cycling conditions:
Spin down the tubes/plate briefly to remove bubbles and place them into the cycler.
denaturation
95°C
5min
denaturation (1)
annealing (2) and extension
95°C
55-68°C
10 sec
30 sec
1)The annealing temperature depends on the melting temperature of the primers used.
2)The elongation time depends on the length of the fragments to be amplified. A time
of 1 min/kb is recommended.
SYBR® is a registered trademark of Invitrogen Corporation, Carlsbad, California, USA
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