Endpoint PCR Green Line


Taq Polymerase GL

2X Master Mix GL

dNTPs and dNTPs mix

Gel Loading Buffer GL

Agarose LE

DNA Ladders GL

2X XtraWhite Master Mix and 2X XtraRTL Master Mix (GeneSpin proprietary formulation), are two premixed, ready-to-use solution containing Xtra-Taq Pol, dNTPs and MgCl2 in a Reaction Buffer optimized for use in PCR amplification of targets present in low copy number and to avoid amplification of non-specific products. Both buffers contain 3.0mM magnesium, PCR enhancers and thickening agents (vortex thoroughly prior to use). 2X Master Mix Standard GL contain an internal fluorescent stain for DNA detection on Agarose gel directly after PCR amplification.

2X XtraRTL Master Mix contains Orange G dye that allows gel loading and electrophoresis of the sample directly from the PCR tube, without further manipulation. 2X XtraWhite Master Mix is supplied with appropriate quantity of 6X Loading Dye. The Orange G dye migrates at the same rate as a duplex DNA fragment of approximately 50 Kbp and does not interfere with DNA migration when it is used as a loading dye for agarose gel electrophoresis.

cat. no.

amount

note

PDF

STS-XMMixW 200* GL

5ml

2X XtraWhite Master Mix GL

STS-XMMixW 1000* GL

25ml

2X XtraWhite Master Mix GL

STS-XMMixRTL 200 GL

5ml

2X XtraRTL Master Mix GL

STS-XMMixRTL 1000 GL

25ml

2X XtraRTL Master Mix GL

*2X XtraWhite Master Mix GL is supplied with appropriate quantity of 6X Loading Dye

FOR RESEARCH USE ONLY

UNIT DEFINITION

One unit is defined as the amount of enzyme required to incorporate 10 nanomoles of dNTPs into acid-insolubile material in 30 min at 74°C.

SHIPPING

Shipped in green ice.

STORAGE

Store at -20C°

SHELF LIFE

12 months

FORM

Liquid

CONCENTRATION

2X conc.

GREEN LINE OVERVIEW

2X Master Mix GL contain an internal fluorescent stain for DNA detection on Agarose gel directly after PCR amplification.

component

primers

DNA Template

MG Water 

2X Master Mix GL

Assay Set-Up:

Before starting, vortex all components thoroughly to ensure homogeneity.

Prepare a premix for the number of assays you need according to the following protocol:

stock conc.

final conc.

2X

1X

15.0ul

1ug/ul each

50ng/ul each

-

-

10-20ng

-

-

30ul reaction

2.0ul each

up to 30ul

1)The annealing temperature depends on the melting temperature of the primers used.

2)The elongation time depends on the length of the fragments to be amplified. A time 

of 1 min/kb is recommended.

Cycling conditions:

Spin down the tubes/plate briefly to remove bubbles and place them into the cycler.

denaturation

denaturation (1)

annealing (2)

extension

95°C

95°C

50-68°C

72°C

5min

30 sec 

30 sec

30 sec 

4 min

GeneSpin Srl

Via Friuli, 51

20135 Milano

P.IVA C.F. 04520270960


Laboratory 

and sales office

c/o Fondazione Filarete

Viale Ortles, 22/4

20139 Milano

tel. +39 02 56660199

fax. +39 02 537250

@ 2008-2023 GeneSpin Srl

www.genespin.com

info@genespin.com

administration@pec.genespin.com

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