2X Quantitative Master Mix SYBR Green, is a 2X premixed, ready-to-use solution (Genespin proprietary formulation) containing Xtra Taq Pol, dNTPs, MgCl2 and stabilizers optimized for use in real time PCR amplification.
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2X Quantitative Master Mix
cat. no.
amount
note
QSTS-MMix200
QSTS-MMix500
5ml
2X conc.
2X conc.
QSTS-MMix1000
25ml
2X conc.
FOR RESEARCH USE ONLY
SHIPPING
Shipped in green ice.
STORAGE
Store at -20C°
avoid freeze/thaw cycles, store dark
Storage at 4 °C for up to 3 months possible.
SHELF LIFE
12 months
FORM
Liquid
CONCENTRATION
2X
12.5ml
MG Water
-
-
> 2X Quantitative Master Mix Probes
qPCR
Assay Set-Up:
Before starting, vortex all components thoroughly to ensure homogeneity.
Prepare a premix for the number of assays you need according to the following protocol:
component
2X Master Mix
primer for
primer rev
DNA Template
stock conc.
2X
10uM
10uM
-
final conc.
1X
100-400 nM
100-400 nM
-
20ul reaction
10.0ul
0.2 - 0.8 ul
0.2 - 0.8 ul
< 500ng
Cycling conditions:
Spin down the tubes/plate briefly to remove bubbles and place them into the cycler.
denaturation
denaturation (1)
annealing (2) and extension
95°C
95°C
55-68°C
5min
10 sec
30 sec
1)The annealing temperature depends on the melting temperature of the primers used.
2)The elongation time depends on the length of the fragments to be amplified. A time
of 1 min/kb is recommended.
50ul reaction
25.0ul
0.5 - 2.0 ul
0.5 - 2.0 ul
<500ng
MG Water
-
-
up to 20ul
up to 50ul
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