2X Optimum qPCR Master Mix is a NEW 2X premixed, ready-to-use solutions (GeneSpin proprietary formulation) containing new S∆Taq Pol, dNTPs, MgCl2 and stabilizers optimized for use in real time PCR amplification of DNA or cDNA.

2X Optimum Master Mixes offers more stability, reproducibility and tolerance to PCR inhibitors, and contain fluorescein for dynamic well factor collection. The addiction of fluorescein has no effect on the PCR reaction efficiency or sensitivity of detection.

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Optimum qPCR Master Mix

cat. no.

amount

note

QSTS-OSMMix200

QSTS-OSMMix500

5ml

2X conc.

2X conc.

MB_qPCR_OptSYBR_files/2X%20Optimum%20SYBR.pdf
.PDFMB_qPCR_OptSYBR_files/2X%20Quantitative%20MMix%20SYBR.pdf

QSTS-OSMMix1000

25ml

2X conc.

FOR RESEARCH USE ONLY

SHIPPING

Shipped in green ice.

STORAGE

Store at -20C°

avoid freeze/thaw cycles, store dark

Storage at 4 °C for up to 3 months possible.

SHELF LIFE

12 months

FORM

Liquid

CONCENTRATION

2X

12.5ml

MG Water

-

-

> 2X Optimum qPCRMaster Mix with SYBR® Green I

qPCR

Assay Set-Up:

Before starting, vortex all components thoroughly to ensure homogeneity.

Prepare a premix for the number of assays you need according to the following protocol:

component

2X Master Mix

primer for

primer rev

DNA Template

stock conc.

2X

10uM

10uM

-

final conc.

1X

100-400 nM

100-400 nM

-

20ul reaction

10.0ul

0.2 - 0.8 ul

0.2 - 0.8 ul

< 500ng

Cycling conditions:

Spin down the tubes/plate briefly to remove bubbles and place them into the cycler.

denaturation

denaturation (1)

annealing (2) and extension

95°C

95°C

55-68°C

5min

10 sec

30 sec

30-45x
1x

1)The annealing temperature depends on the melting temperature of the primers used.

2)The elongation time depends on the length of the fragments to be amplified. A time

of 1 min/kb is recommended.

50ul reaction

25.0ul

0.5 - 2.0 ul

0.5 - 2.0 ul

<500ng

MG Water

-

-

up to 20ul

up to 50ul

melt curve

from 65°C to 95°C

0.5°C/5sec

1x

SYBR® is a registered trademark of Invitrogen Corporation, Carlsbad, California, USA

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20135 Milano

P.IVA C.F. 04520270960

Laboratory

and sales office

c/o Fondazione Filarete

Viale Ortles, 22/4

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fax. +39 02 537250

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