An highly processive, recombinant (from E.coli strain), thermostable DNA with a very high efficiency of 5’- 3’ polymerase activity and 3´- 5’ exonuclease (non-proofreading) activity. Xtra Taq Pol catalyzes the addition of mononucleotide units to the 3’-end of a primer chain, leading to the formation of DNA products that have 3’-overhanging A nucleotides (thus can be used in TA cloning). This enzyme remains funtional even after prolonged incubation steps at 95°C. The enzyme is supplied at 5U/μl and comes with 5X XtraRTL GL (Ready To Load) new buffer. 5X XtraRTL GL Reaction Buffer is a Genespin proprietary formulation, developed for standard and/or high-fidelity amplification of high-GC (>75%) templates. The buffer contain 7.5mM magnesium, PCR enhancers and thickening agents (vortex thoroughly prior to use). 5X XtraRTL GL contain an internal fluorescent stain for DNA detection on Agarose gel directly after PCR amplification.
5X XtraRTL GL Reaction Buffer contains Orange G dye that allows gel loading and electrophoresis of the sample directly from the PCR tube, without further manipulation. The Orange G dye migrates at the same rate as a duplex DNA fragment of approximately 50 Kbp and does not interfere with DNA migration when it is used as a loading dye for agarose gel electrophoresis.
