2X Hot Start XtraWhite Master Mix GL and 2X Hot Start XtraRTL Master Mix GL (Genespin proprietary formulation), are two premixed, ready-to-use solution containing Hot Taq Pol, dNTPs and MgCl2 in a Reaction Buffer optimized for use in PCR amplification of targets present in low copy number and to avoid amplification of non-specific products. Both buffers contain 3.0mM magnesium, PCR enhancers and thickening agents (vortex thoroughly prior to use). 2X Master Mix HotStart GL contain an internal fluorescent stain for DNA detection on Agarose gel directly after PCR amplification. Both Master Mixes allow for specific PCR amplification by keeping the enzyme inactive until the temperature reaches approximately 40°C, while also reducing samples preparation time as well as risk of contamination from  multiple pipetting steps.

2X Hot Start XtraRTL Master Mix GL contains Orange G dye that allows gel loading and electrophoresis of the sample directly from the PCR* tube, without further manipulation. 2X Hot Start XtraWhite Master Mix GL is supplied with appropriate quantity of 6X Loading Dye. The Orange G dye migrates at the same rate as a duplex DNA fragment of approximately 50 Kbp and does not interfere with DNA migration when it is used as a loading dye for agarose gel electrophoresis.

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You are here: molecular biology / endpoint PCR green line /  2X Master Mixes HotStart GL

Master mixes

cat. no.

amount

note

STS-HXMMixW 200* GL

STS-HXMMixW 1000* GL

5ml

2X Hot Start XtraRTL Master Mix GL

2X Hot Start XtraRTL Master Mix GL

.PDFMB_end_point_MMIX_Hot_Start_GL_files/StoS%202X%20Master%20Mix%20Hot%20Start%20GL%20ds.pdf

STS-HXMMixRTL 200 GL

STS-HXMMixRTL 1000 GL

25ml

5ml

2X Hot Start XtraRTL Master Mix GL

2X Hot Start XtraRTL Master Mix GL

FOR RESEARCH USE ONLY

SHIPPING

Shipped in green ice.

STORAGE

Store at -20C°, avoid freeze/thaw cycles, store dark.

SHELF LIFE

12 months

FORM

Liquid

CONCENTRATION

2X conc.

Assay Set-Up:

Before starting, vortex all components thoroughly to ensure homogeneity.

Prepare a premix for the number of assays you need according to the following protocol:

component

2X Master Mix

primers

DNA Template

MG Water

25ml

stock conc.

2X

1ug/ul each

-

-

final conc.

1X

50ng/ul each

-

-

30ul reaction

15.0ul

2.0ul each

10-20ng

up to 30ul

> 2X Master mix HotStart GL

Cycling conditions:

Spin down the tubes/plate briefly to remove bubbles and place them into the cycler.

denaturation

denaturation (1)

annealing (2)

extension

95°C

95°C

50-68°C

72°C

5min

30 sec

30 sec

30 sec

4 min

20-35x
1x

1)The annealing temperature depends on the melting temperature of the primers used.

2)The elongation time depends on the length of the fragments to be amplified. A time

of 1 min/kb is recommended.

*2X Hot Start XtraWhite Master Mix GL is supplied with appropriate quantity of 6X Loading Dye GL

endpoint PCR

green line

GREEN LINE OVERVIEW

2X Master Mix HotStart GL contain an internal fluorescent stain for DNA detection on Agarose gel directly after PCR amplification.

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