> Protein A Agarose

Protein A Resin is useful for affinity purification and isolation of IgG. Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly through Fc regions. Native Protein A has 5 IgG binding domains and many others domains of unknown functions. Recombinant Protein A contains mainly five high affinity (Ka=108/M) IgG binding domains with others non essential domains removed to reduce nonspecific binding. Additionally a 3×Cys tag was engineered to the C-terminal of rec-protein A to facilitate its immobilization or conjugation.