GeneSpin Srl
Via Friuli, 51
20135 Milano
P.IVA C.F. 04520270960
Laboratory
and sales office
c/o Fondazione Filarete
Viale Ortles, 22/4
20139 Milano
tel. +39 02 56660199
fax. +39 02 537250
2X XtraWhite Master Mix and 2X XtraRTL Master Mix (GeneSpin proprietary formulation), are two premixed, ready-to-use solution containing Xtra-Taq Pol, dNTPs and MgCl2 in a Reaction Buffer optimized for use in PCR amplification of targets present in low copy number and to avoid amplification of non-specific products. Both buffers contain 3.0mM magnesium, PCR enhancers and thickening agents (vortex thoroughly prior to use).
2X XtraRTL Master Mix contains Orange G dye that allows gel loading and electrophoresis of the sample directly from the PCR tube, without further manipulation. 2X XtraWhite Master Mix is supplied with appropriate quantity of 6X Loading Dye. The Orange G dye migrates at the same rate as a duplex DNA fragment of approximately 50 Kbp and does not interfere with DNA migration when it is used as a loading dye for agarose gel electrophoresis.
STS-XMMixW 200*
5ml
2X XtraWhite Master Mix
STS-XMMixW 1000*
25ml
2X XtraWhite Master Mix
STS-XMMixRTL 200
5ml
2X XtraRTL Master Mix
STS-XMMixRTL 1000
25ml
2X XtraRTL Master Mix
*2X XtraWhite Master Mix is supplied with appropriate quantity of 6X Loading Dye
FOR RESEARCH USE ONLY
UNIT DEFINITION
One unit is defined as the amount of enzyme required to incorporate 10 nanomoles of dNTPs into acid-insolubile material in 30 min at 74°C.
SHIPPING
Shipped in green ice.
STORAGE
Store at -20C°
SHELF LIFE
12 months
FORM
Liquid
CONCENTRATION
2X conc.
component
primers
Assay Set-Up:
Before starting, vortex all components thoroughly to ensure homogeneity.
Prepare a premix for the number of assays you need according to the following protocol:
stock conc.
final conc.
2X Master Mix
2X
1X
1ug/ul each
50ng/ul each
DNA Template
-
-
30ul reaction
15.0ul
2.0ul each
10-20ng
Cycling conditions:
Spin down the tubes/plate briefly to remove bubbles and place them into the cycler.
denaturation
denaturation (1)
annealing (2)
extension
95°C
95°C
50-68°C
72°C
5min
30 sec
30 sec
30 sec
4 min
MG Water
-
-
up to 30ul
1)The annealing temperature depends on the melting temperature of the primers used.
2)The elongation time depends on the length of the fragments to be amplified. A time
of 1 min/kb is recommended.
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